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Image Search Results
Journal: Animals : an Open Access Journal from MDPI
Article Title: ATP Induces Interleukin-8, Intracellular Calcium Release, and ERK1/2 Phosphorylation in Bovine Endometrial Cells, Partially through P2Y Receptors
doi: 10.3390/ani13050841
Figure Lengend Snippet: ATP increases interleukin-8 (IL-8) production in BEND cells. ( A ) BEND cells were treated with vehicle (PBS) or different concentrations of ATP (0.1, 1, 10, 50, and 100 μM) for 24 h, and IL-8 production was assessed in the culture medium by ELISA assay. LPS (500 ng/mL) was used as a positive control. ( B ) BEND cells were incubated with vehicle (PBS) or ATP (10, 50, and 100 μM) without or with LPS (500 ng/mL) for 24 h, and IL-8 production was assessed in the culture medium by ELISA assay. ( C ) BEND cells were incubated with vehicle (DMSO 0.1%) or suramin (50 μM) for 15 min, and then ATP (50 μM) was added for 24 h, and IL-8 production was determined by ELISA assay. n = 3 independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.
Article Snippet: BEND cells (n = 3 independent experiments) were cultured with
Techniques: Enzyme-linked Immunosorbent Assay, Positive Control, Incubation
Journal: Animals : an Open Access Journal from MDPI
Article Title: ATP Induces Interleukin-8, Intracellular Calcium Release, and ERK1/2 Phosphorylation in Bovine Endometrial Cells, Partially through P2Y Receptors
doi: 10.3390/ani13050841
Figure Lengend Snippet: ATP increases intracellular calcium mobilization and ERK1/2 phosphorylation. ( A ) Fura 2AM-loaded BEND cells were treated with vehicle (PBS) and basal fluorescence was registered for 60 s; then, different concentrations of ATP (0.1, 1, or 50 μM) were added and the fluorescence was determined for 240 s. RFU = relative fluorescence units. ( B ) Fura 2AM-loaded BEND cells were incubated with vehicle (0.1% DMSO) or suramin (10, 50, or 100 μM) for 15 min. Then, the basal fluorescence was registered for 60 s and ATP (50 μM) was added, and the fluorescence registered for 140 s. V = vehicle. ( C ) BEND cells were incubated with vehicle (0.1% DMSO) or suramin (50 μM) for 15 min, and then vehicle (PBS) or ATP (50 μM) was added, and incubation was carried out for 5 min. Total proteins were analyzed by immunoblot with antibodies against phospho-ERK1/2 and total ERK1/2. c = control (0.1% DMSO). S = Suramin. Bands’ intensities are shown as the ratio phospho-ERK1/2/ERK1/2 in the bar graph. ( D ) BEND cells were incubated with vehicle (0.1% DMSO) or suramin (1, 10, 100, or 300 μM) for 30 min or 24 h, and the cellular viability was measured by propidium iodide assay. * p < 0.05, ** p < 0.01 compared with the control. Images are representative of three independents experiments.
Article Snippet: BEND cells (n = 3 independent experiments) were cultured with
Techniques: Fluorescence, Incubation, Western Blot